Parathyroid hormone (PTH) exerts classical actions on bone and mineral metabolism by activating PTH/PTHrP receptors (PTH1Rs) on target cells, including osteoblasts and osteocytes in bone. Such bone cells also express an additional receptor for PTH distinct from PTH1Rs, that recognize determinants within the carboxyl (C)-terminal portion of PTH (1-84), the C-terminal PTH receptor (“CPTHR”). CPTHRs previously were found to regulate intercellular communication, cell survival and to increase cytosolic calcium in bone cells in a manner dependent upon voltage-sensitive calcium channels. As intracellular free calcium is known to regulate cytoskeletal function, we sought to determine if CPTHR activation altered cytoskeletal structure in OC-59 osteocytic cells, which lack PTH1Rs but express abundant CPTHRs.
Treatment of OC-59 cells with 100 nM hPTH (53-84) for 10 minutes induced marked condensations of the cytoskeletal components actin and vinculin, as visualized by immunofluorescence in permeabilized cells. This effect was not observed in cells treated with vehicle alone or with the CPTHR ligand for only 2 minutes. These changes also were not seen in cells exposed for 10 minutes to the inactive CPTH analog, [Ala55-57]PTH (53-84), which does not bind to CPTHRs and does not induce a calcium signal in OC-59 cells. Cytoskeletal condensation induced by hPTH (53-84) was blocked by pretreatment with gadolinium chloride, which is known to inhibit CPTHR-dependent calcium responses in these cells. Taken together, these results suggest that calcium influx induced by CPTHR activation may play an important role in regulating the cytoskeleton in osteocytes.
"Open access will revolutionize 21st century knowledge work and accelerate the diffusion of ideas and evidence that support just in time learning and the evolution of thinking in a number of disciplines."
"It is important that students and researchers from all over the world can have easy access to relevant, high-standard and timely scientific information. This is exactly what Open Access Journals provide and this is the reason why I support this endeavor."
"Publishing research articles is the key for future scientific progress. Open Access publishing is therefore of utmost importance for wider dissemination of information, and will help serving the best interest of the scientific community."
"Open access journals are a novel concept in the medical literature. They offer accessible information to a wide variety of individuals, including physicians, medical students, clinical investigators, and the general public. They are an outstanding source of medical and scientific information."
"Open access journals are extremely useful for graduate students, investigators and all other interested persons to read important scientific articles and subscribe scientific journals. Indeed, the research articles span a wide range of area and of high quality. This is specially a must for researchers belonging to institutions with limited library facility and funding to subscribe scientific journals."
"Open access journals represent a major break-through in publishing. They provide easy access to the latest research on a wide variety of issues. Relevant and timely articles are made available in a fraction of the time taken by more conventional publishers. Articles are of uniformly high quality and written by the world's leading authorities."
"Open access journals have transformed the way scientific data is published and disseminated: particularly, whilst ensuring a high quality standard and transparency in the editorial process, they have increased the access to the scientific literature by those researchers that have limited library support or that are working on small budgets."
"Not only do open access journals greatly improve the access to high quality information for scientists in the developing world, it also provides extra exposure for our papers."
"Open Access 'Chemistry' Journals allow the dissemination of knowledge at your finger tips without paying for the scientific content."
"In principle, all scientific journals should have open access, as should be science itself. Open access journals are very helpful for students, researchers and the general public including people from institutions which do not have library or cannot afford to subscribe scientific journals. The articles are high standard and cover a wide area."
"The widest possible diffusion of information is critical for the advancement of science. In this perspective, open access journals are instrumental in fostering researches and achievements."
"Open access journals are very useful for all scientists as they can have quick information in the different fields of science."
"There are many scientists who can not afford the rather expensive subscriptions to scientific journals. Open access journals offer a good alternative for free access to good quality scientific information."
"Open access journals have become a fundamental tool for students, researchers, patients and the general public. Many people from institutions which do not have library or cannot afford to subscribe scientific journals benefit of them on a daily basis. The articles are among the best and cover most scientific areas."
"These journals provide researchers with a platform for rapid, open access scientific communication. The articles are of high quality and broad scope."
"Open access journals are probably one of the most important contributions to promote and diffuse science worldwide."
"Open access journals make up a new and rather revolutionary way to scientific publication. This option opens several quite interesting possibilities to disseminate openly and freely new knowledge and even to facilitate interpersonal communication among scientists."
"Open access journals are freely available online throughout the world, for you to read, download, copy, distribute, and use. The articles published in the open access journals are high quality and cover a wide range of fields."
"Open Access journals offer an innovative and efficient way of publication for academics and professionals in a wide range of disciplines. The papers published are of high quality after rigorous peer review and they are Indexed in: major international databases. I read Open Access journals to keep abreast of the recent development in my field of study."
"It is a modern trend for publishers to establish open access journals. Researchers, faculty members, and students will be greatly benefited by the new journals of Bentham Science Publishers Ltd. in this category."