The Open Leukemia Journal


ISSN: 1876-8164 ― Volume 5, 2013

Establishment of an Adult T-Cell Leukemia Cell Line (HU-ATTAK) Dependent for Proliferation on Human Umbilical Cord Vein Endothelial Cells

Yoshitoyo Kagami*, 1, 5, Karnan Sivasundaram3, Aya nakagawa3, Kazuiku Oshiro4, Harumi Kato3, Koichi Koike2, Yasuo Morishima1 , Masao Seto*, 3, 6
1 Department of Hematology and Cell Therapy,
2 Department of Clinical Laboratories, Aichi Cancer Center Hospital,
3 Division of Molecular Medicine, Aichi Cancer Center Research Institute,
4 Department of Hematology, Okinawa Prefectural Nanbu Medical Center & Children’s Medical Center,
5 Department of Hematology, Toyota Kosei Hospital
6 Department of Cancer Genetics, Nagoya University Graduate School of Medicine, Japan


A novel cell line, HU-ATTAK, was established from the peripheral blood of a patient with adult T-cell leukemia by means of co-culture with human umbilical cord vein endothelial cells (HUVEC) in the presence of interleukin-2 and damnacanthal. Immunophenotypic and cytogenetic analyses showed that HU-ATTAK is originally derived from leukemic cells and maintains the typical features of adult T-cell leukemia-lymphoma (ATLL). The growth of HU-ATTAK depends on IL-2 and HUVEC. When IL-2 was removed from the culture, HU-ATTAK stopped growing and eventually died through apoptosis. On the other hand, when HUVEC was removed from the culture, HU-ATTAK ceased proliferation but survived for a period of over two weeks. A culture experiment using micropore membranes showed that attachment of HU-ATTAK to HUVEC is necessary for proliferation. Stimulation of the co-stimulatory molecule OX40 ligand, which is expressed on HUVEC, is also involved in HU-ATTAK growth because the anti-OX40 ligand antibody completely inhibited the growth. These findings suggest the importance of the microenvironment for the survival and proliferation of some cases of ATLL. The HU-ATTAK cell line may thus be useful for studying the growth mechanism of ATLL and might yield new insights into the pathogenesis of ATLL.

Keywords: Adult T-cell leukemia/lymphoma, Human umbilical cord vein endothelial cells, OX40, cell line.

Article Information

Identifiers and Pagination:

Year: 2011
Volume: 4
First Page: 1
Last Page: 8
Publisher Id: TOLEUKEMIAJ-4-1
DOI: 10.2174/1876816401104010001

Article History:

Received Date: 3/1/2011
Revision Received Date: 26/1/2011
Acceptance Date: 31/1/2011
Electronic publication date: 9/3/2011
Collection year: 2011

© Ahlberg etal.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: // which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to these authors at the (YK) Department of Hematology, Toyota Kosei Hospital, 500-1, Ibohara, Josui-cho, Toyota, 470-0396, Japan; Tel: (+81) - 565-43-5000; Fax: (+81) - 565-43-5100; and (MA) Division of Molecular Medicine, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan; Tel: (+81)-52-762-6111; Fax: (+81)-52-763-5233; E-mail:

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