The Open Medicinal Chemistry Journal

ISSN: 1874-1045 ― Volume 15, 2021

Study on the Interaction of 4'-Hydroxychalcones and their Mannich Derivatives with Calf Thymus DNA by TLC and Spectroscopic Methods, a DNA Cleavage Study

Zsuzsanna Rozmer1, Aline Bernardes2, 3, Caridad N. Pérez3, Pál Perjési1, *
1 Institute of Pharmaceutical Chemistry, University of Pécs, Rókus str. 2., H-7624 Pécs, Hungary
2 Department of Education, Research and Extension, Federal Institute of Education, Science and Technology of Mato Grosso, 78050-560, Cuiabá-MT, Brazil
3 Institute of Chemistry, Federal University of Goiás, 74690-900 Goiánia-GO, Brazil



Phenolic Mannich bases derived from hydroxychalcones show remarkable cytotoxic potencies towards cancer cell lines. However, the exact mechanism of action is still partially uncleared.


Interaction of two hydroxychalcones and their Mannich derivatives with calf thymus DNA (ctDNA) has been investigated.


Thin-layer chromatography and UV-Vis spectroscopic method were used for studying the interaction. The binding constant has been determined by UV-Vis spectrophotometric titration. The DNA cleavage activity of the compounds was studied by agarose gel electrophoresis.


Interaction of the compounds with ctDNA exhibited relatively high intrinsic binding constant (4-5x104 M-1). The results indicate existence of weak, non-covalent interactions between the investigated derivatives with ctDNA. Some compounds showed a slight DNA cleavage activity with pBR322.


The obtained results provide additional knowledge on the previously documented cytotoxicity against tumor cell lines of the hydroxychalcones and their Mannich-derivatives.

Keywords: Chalcones, Hydroxychalcones, Mannich bases, DNA binding, UV-Vis spectroscopy, DNA cleavage.

Article Information

Identifiers and Pagination:

Year: 2020
Volume: 14
First Page: 122
Last Page: 131
Publisher Id: TOMCJ-14-122
DOI: 10.2174/1874104502014010122

Article History:

Received Date: 06/06/2020
Revision Received Date: 03/09/2020
Acceptance Date: 07/09/2020
Electronic publication date: 27/11/2020
Collection year: 2020

© 2020 Rozmer et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Institute of Pharmaceutical Chemistry, University of Pécs, Rókus str. 2., H-7624Pécs, Hungary; E-mail:

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