RESEARCH ARTICLE
False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients
Silje V. Hoegh, Charlotte N. Agergaard*, Marianne N. Skov, Michael Kemp
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
First Page: 51
Last Page: 54
Publisher ID: TOMICROJ-13-51
DOI: 10.2174/1874285801913010051
Article History:
Received Date: 31/10/2018Revision Received Date: 28/01/2019
Acceptance Date: 06/02/2019
Electronic publication date: 28/02/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis.
Objective:
The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR.
Methods:
Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001.
Results:
The results showed that 2.3% of the samples were false-positive for Bordetella pertussis.
Conclusion:
In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.