False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients
Silje V. Hoegh, Charlotte N. Agergaard*, Marianne N. Skov, Michael Kemp
Department of Clinical Microbiology, Odense University Hospital, Winsløwparken 21, 2, DK-5000 Odense, Denmark.
Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis.
The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR.
Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001.
The results showed that 2.3% of the samples were false-positive for Bordetella pertussis.
In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
* Address correspondence to this author at the Department of Clinical Microbiology, Odense University Hospital, Winsløwparken 21, 2, DK-5000 Odense C, Denmark; Tel: +45 6541 1347; E-mail: email@example.com