RESEARCH ARTICLE


False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients



Silje V. Hoegh, Charlotte N. Agergaard*, Marianne N. Skov, Michael Kemp
Department of Clinical Microbiology, Odense University Hospital, Winsløwparken 21, 2, DK-5000 Odense, Denmark.


Article Metrics

CrossRef Citations:
1
Total Statistics:

Full-Text HTML Views: 4799
Abstract HTML Views: 2314
PDF Downloads: 1084
ePub Downloads: 802
Total Views/Downloads: 8999
Unique Statistics:

Full-Text HTML Views: 2309
Abstract HTML Views: 1175
PDF Downloads: 683
ePub Downloads: 499
Total Views/Downloads: 4666



Creative Commons License
© 2019 Hoegh et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Clinical Microbiology, Odense University Hospital, Winsløwparken 21, 2, DK-5000 Odense C, Denmark; Tel: +45 6541 1347; E-mail: charlotte.agergaard@rsyd.dk


Abstract

Background:

Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis.

Objective:

The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR.

Methods:

Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001.

Results:

The results showed that 2.3% of the samples were false-positive for Bordetella pertussis.

Conclusion:

In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

Keywords: Bordetella pertussis, Real-time PCR, IS481, IS1002, IS1001, False-positive.