351 EML, NDSU Dept. #
2620, PO Box 6050, North Dakota State University, Fargo, ND, 58108-
6050; Department of Health, Nutrition and Exercise Sciences, North Dakota
State University, Fargo, North Dakota.
The objective of this study was to determine if flaxseed lignan (secoisolariciresinol diglucoside, SDG) would
improve glucose uptake by human red blood cells (RBCs). To measure glucose uptake of RBCs, the RBCs were
pretreated, in vitro, with SDG (0, 10, 50, or 100 µM) and then were incubated with or without insulin (100 µU/ml). After
incubation, 3-O-(3H-Methyl)-D-glucose uptake by the RBCs was measured. RBCs pretreated with 50 or 100 µM SDG
showed an increase in glucose uptake compared to control (0 µM SDG) or RBCs pretreated with 10 µM SDG (p<0.0001).
In addition, glucose uptake was increased in RBCs with insulin stimulation compared to RBCs without insulin stimulation
(p<0.0001). An interaction between SDG and insulin was also observed (p<0.0001). Pretreatment of RBCs with SDG
(50 or 100 µM) and insulin increased glucose uptake compared to those without insulin stimulation (p<0.0001). The
results indicate potential beneficial effects of SDG on glycemic control by increasing glucose uptake of RBCs.