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Abstract HTML Views: 222 PDF Downloads: 65 Total Views/Downloads: 287
There is evidence that the antioxidant systems are imbalanced in hypercholesterolemic rats due to
oxidative stress. Dietary supplementation of Pleurotus sajor-caju and P. florida are anti-hypercholesterolemic in rats. In
this study, it was investigated whether these mushrooms have any effects on the antioxidant systems in
hypercholesterolemic rat liver, which are altered.
Twenty young Long-Evans rats were randomly divided into 4 groups: control (fed basal diet), HC (fed 1% cholesterol
with basal diet), HC+PS (fed 5% P. sajor-caju powder with 1% cholesterol and basal diet) and HC+PF (fed 5% P. florida
powder with 1% cholesterol and basal diet). Rats were fed for 40 days, and then after sacrifice livers were collected, washed
and homogenized. The content of reduced glutathione (GSH), thiobarbituric acid reactive substance (TBARS), and the
activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) in homogenate were measured.
All the studied parameters were altered in hypercholesterolemic rats significantly in comparison with control
group, except catalase (non-significant). Feeding of P. florida protected liver lipid peroxidation in hypercholesterolemic
rat, as TBARS was significantly lower (by 34.5%) in HC+PF group than in HC group. Feeding of both mushrooms
showed significant improvement in the activity of GPx in liver of hypercholesterolemic rats (in HC+PS and HC+PF, the
activity was higher by 50% and 80%, respectively than in HC group). However, mushroom feeding showed no significant
effect on GSH level, SOD and catalase activity in hypercholesterolemic rats.
This investigation confirms the antioxidant activities of P. sajor-caju and P. florida to some extent in
hypercholesterolemic rats in addition to their anti-hypercholesterolemic activity.