The objective of this study is to establish a quantitative method for simultaneous determination of costunolide
and dehydrocostuslactone in Xin-ke-shu preparations. The HPLC quantitative analysis was established on a Hypersil BDS
column (100x 4.6 mm, 2.4 μm, Thermo) with UV detection at 225 nm. The mobile phase was water and methanol (30:70,
v/v), and used at a flow rate at 0.5 mL·min- 1 . The established method showed a good linearity (R2 > 0.9999) over the
investigated concentration ranges (0.07-2.80 μg), good inter-day and intra-day precisions (less than 3%) and good
recoveries (from 99.82% to 99.98%) for both target compounds. The method was found to be suitable for simultaneous
determination of costunolide and dehydrocostuslactone in Xin-ke-shu preparations and can be used as a comprehensive
approach to the previous reported quality control of Xin-ke-shu preparations.