The antioxidant, hepatoprotective, and anthelmintic activities of methanol extract of leaves of Urtica dioica L.
(MEUD) were investigated in the present study. Hepatoprotective potential of the extract was evaluated against CCl4–
induced hepatic injury in Wister rats. In addition, reducing power assay, determination of total antioxidant activity, DPPH
radical scavenging activity and nitric oxide scavenging activity were investigated to evaluate the in vitro antioxidant activity
of the extract. The results indicated significant antioxidant activity of the extract comparable to standard antioxidant
compounds like α-tocopherol, ascorbic acid and BHA (Butylated hydroxyanisole). Significant hepatoprotective profile
was also observed with maximum hepatoprotection at the dose of 400 mg/kg as suggested by decreased level of serum
ALT, AST, ALP and total bilirubin levels towards normalization. Pretreatment of animals with the extract at all the dose
level (100, 200, and 400 mg/kg) resulted a significant decrease in malonyldehyde (MDA) level as well as a significant increase
in superoxide dismutase (SOD) level clearly indicating inhibition of lipid peroxidation and improvement of antioxidant
defense enzyme system. Anthelmintic activity of the methanol extract was also investigated using adult Indian
earth worms (Pheretima posthuma) and the results revealed a dose dependent increase in anthelmintic activity of the extract
at 25, 50, and 100 mg/mL concentrations.