Pharmaceutical Crops


ISSN: 2210-2906 ― Volume 6, 2017

Assessment of Chemopreventive Contents of Native American Juneberries (Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem.)

Shimara Gunawardana1, Keeli Eberhart1, Kerry Hartman2, Fathi T. Halaweish1, *
1 Department of Chemistry & Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007, USA
2 Nueta Hidatsa Sahnish College, PO Box 490, New Town, ND 58763


Juneberry (Amelanchier alnifolia) is a shrub with edible berry-like fruit commonly found across the Northern Great Plains of North America and widely used by Native Americans as a medicinal plant. This study was an effort to assess the chemopreventive contents of juneberry extracts in ethanol by measuring the total phenolic content, orthophenolic content, DPPH radical scavenging activity, ABTS radical cation decolorization assay, and cytoprotective effects on HepG2 cells. The total phenolic content upon treatment with Folin-Ciocalteu’s reagent ranged from 0.2261 ± 0.0031 to 19.311 ± 1.651 mg.g-1 tannic acid equivalents. Orthophenolic content quantified by monitoring the bathochromic shifts upon reacting with sodium molybdate ranged from 0.12 ± 0.03 to 2.38 ± 0.15 mg.g-1 of FW. Antioxidant activity using DPPH assay ranged from 50.08 ± 0.77 to 98.60 ± 1.12%, in contrast to quercetin dihydrate with an activity of 86.20 ± 0.05%. ABTS radical scavenging assay produced inhibition from 1.90 ± 0.11 to 60.25 ± 1.65% as opposed to 6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid (Trolox) with an inhibition of 94.03 ± 0.21% . HepG2 cells treated with 100 µg/µL of sample WC2B showed 56% cell-viability against tert-Butyl hydroperoxide in comparison to quercetin dihydrate which showed 90% cytoprotection. Total phenolic content and orthophenolic content in juneberries from 2012 were higher than in juneberries obtained from the 2013 and 2014. Similarly fruit grown in the wild showed significantly higher antioxidant activity and phenolic content than those grown in domestic cultivars.

Keywords: Juneberry, Amelanchier alnifolia, Chemopreventive, Antioxidant activity, Native American, Cytoprotection, phenolics, DPPH, ABTS, Orthophenolic, TBHP.

Article Information

Identifiers and Pagination:

Year: 2016
Volume: 6
First Page: 13
Last Page: 21
Publisher Id: TOPHARMCJ-6-13
DOI: 10.2174/2210290601606010013

Article History:

Received Date: 22/02/2016
Revision Received Date: 20/09/2016
Acceptance Date: 25/10/2016
Electronic publication date: 27/12/2016
Collection year: 2016

© Gunawardana et al. ; Licensee Bentham Open

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Chemistry & Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007, USA; E-mail:

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