Many existing pharmaceuticals are rendered ineffective in the treatment of central nervous system (CNS) diseases due to the highly challenging aspects of drug delivery to the most delicate organ namely brain. In order to overcome the problems of delivering neuroprotective agents to the CNS, numerous strategies have been proposed. Among the developed drug carrier to the CNS, poly lactic-co-glycolic acid (PLGA) microspheres have shown desirable outcomes because of their biocompatibility, biodegradability, convenient processability and resorbability through natural pathways. Meanwhile, a comprehensive understanding of the factors affecting drug release mechanisms from microspheres is critical to the design of optimal drug-loaded microparticles. In the present study, we investigated the physicochemical and emulsifying properties of synthesized L-dopa-loaded microsphere. A series of microspheres of different compositions were prepared by varying the salt (NaCl) content, stabilizer content and homogenizer speed. The prepared microspheres were loaded with L-dopa and characterized by SEM techniques to gain insights into the structural and morphological features. The microspheres size was also determined to elucidate the influence of varying formulation and dynamic properties on the drug release pattern. After evaluating morphology and size of the microspheres, the optimum formulation and process parameters including speed of stirring applied for emulsification, drug concentration, amounts of surfactant and NaCl content in the solvent, were revealed using taguchi software according to the prolonged drug release pattern of microspheres.