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The aim of the study was to investigate the effect of supplemental probiotic preparation on performance indices
and serum biochemistry of broiler chickens. Two hundred day-old Marshall broiler chicks were randomly selected and
distributed based on the level of supplementation into four groups of 50 chicks each (Control, C; E10.5%, E21.5% and
E32.0%). Chicks were fed commercial broiler starter diet for the first 28 days of age followed by pelleted finisher diet
from 29-42 days. Chickens fed 2.0% probiotic had a significantly higher body weight when compared with the control
group. Activity of alanine aminotransferase differed significantly in the group E1 and especially (p<0.01) in the group E2.
Alkaline phosphatase activity decreased significantly (p<0.05), when compared with that of the control group. Blood
glucose concentration was significantly different (p<0.05) in E2 probiotic supplemented group when compared with
control. Cholesterol concentration in broiler chickens decreased significantly (p<0.01) in E1 experimental group.