Dichloromethane extract of the Vitex Rotundifolia stems was subjected to in vitro anti-inflammatory tests using xanthine oxidase inhibitory assay and lipoxygenase inhibition assay. The extract showed 54.6% inhibition against xanthine oxidase using NDGA as a positive control however did not exhibit any inhibitory activity against lipoxigenase. Further separation of the extract successfully yielded two aldehydic compounds identified as 6-hydroxy-4-(4’-hydroxy-3’-methoxyphenyl)-3- hydroxymethyl-7-methoxy-3,4-dihydro-2-napthaldehyde and 7,8-dimethoxy-4-(4’-hydroxy-3’-methoxyphenyl)-3-hydroxymethyl- 3,4-dihydro-2-napthaldehyde. Both compounds were isolated after repeated column chromatography using DCM:EtOAc:MeOH (9:0.5:0.5) as solvent system and further purified using Preparative Thin Layer Chromatography (PTLC). Structure elucidation was accomplished using spectroscopic methods, mostly Nuclear Magnetic Resonance spectroscopy.