In Malaysia, there about 15 genera and 212 species of Lauraceae family and one of the species that reported contained various
aporphine and bisbenzylisoquinoline types alkaloids is Alseodaphne corneri Kosterm. The plant of this family growth in
moderate size in Singapore, Malaysia, Jawa, Sumatra and Borneo. The phytochemical study of the roots of Alseodaphne
corneri (Lauraceae) had been carried out. Chromatographic separation of the alkaloid extract led to the isolation of four
isoquinoline alkaloids namely laetanine (1), boldine (2), O-methyllimacusine (3) and stephasubine (4). The isolation and
purification of the alkaloids were achieved using column chromatography (CC) and preparative thin layer chromatography
(PTLC). The structural elucidation was performed by spectral methods mainly UV, IR, NMR including 1D-NMR (1H and 13C)
and 2D-NMR (COSY, HMQC and HMBC).