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Thrombosis and cardiovascular-related diseases can be caused by fibrin aggregation. Thrombolytic agents have been used to
treat thrombolytic problems and cardiovascular-related diseases. There is a need to search for new thrombolytic agents from
bacteria, fungi, insects and fermented foods. To name a few, recently, fungi such as Aspergillus oryzae KSK-3, Fusarium sp.
CPCC 480097 and Fusarium sp. BLB were found to be able of producing protease fibrinolytic enzyme. Potential protease
fibrinolytic enzymes were observed from Lignosus rhinocerus collected from the forest of Sg Perak. Out of the 6 sclerotia
obtained, one of them named LR-1 produced the maximum clear zone on Skim Milk Agar. LR-1 was then tested on fibrin plate
incubated at 37°C for 18 hours and produced a clear zone as compared to positive control, plasmin. Leaves of Hibiscus sp. were
wiped with 70% alcohol and 70% sodium hypochlorite for sterile disinfectant. The leaves were then cut into pieces of 4cm and
placed on Potato Dextrose Agar. Fungi grown from the leave pieces were sub cultured onto a new Potato Dextrose Agar.16
endophytic fungi were isolated from Hibiscus sp. leaves from six locations in Shah Alam, Selangor. Four isolated endophytic
fungi showed positive protease activity on Skim Milk Agar.