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Pereskia bleo, locally known as Jarum Tujuh Bilah, has been claimed to have various medicinal capabilities, including anticancer,
anti-rheumatic, and anti-inflammatory activities. In Malaysia, P.bleo has been used as a natural remedy either eaten raw
(leaves) or taken as a concoction brewed from dried plant. Hot water extraction method was selected in the present study to
mimic the traditional preparation. The hot water extract was then subjected to fractionation process prior to bioassays. Results
of exogenous antioxidant assays, including the Total Phenolic content, DPPH and ABTS assays elucidated that hot water
extract of P.bleo and its fractions have moderate antioxidant activities, with ethyl acetate (EA) fraction exhibited the strongest
antioxidant activity. Cytotoxicity of P.bleo extracts towards various cancer cell lines were tested using MTT assays. MTT
results showed that P.bleo extracts possessed moderate cytotoxicity towards tested cell lines, with EA fraction showing the
lowest IC50 value (104.47±3.5 μg/ml) towards HT-29 cell line. EA fraction was shown to induce apoptosis in HT-29 cell line.
The flow cytometric data of Annexin V-FITC/PI staining assay suggested that EA fraction was able to induce early and late
apoptosis in HT-29 cells in a dose-dependent pattern. Meanwhile, in DNA fragmentation assay, DNA ladder was apparent in
HT-29 cells following treatment with EA fraction, indicating the occurrence of late apoptosis.