Abstract HTML Views: 267 PDF Downloads: 144 Total Views/Downloads: 411
Abstract HTML Views: 174 PDF Downloads: 110 Total Views/Downloads: 284
A rapid decline in research and development on new antibiotics coincides with increasing frequency of infections caused by multidrug-
resistant pathogens. The key reason of bacterial resistance is the indiscriminate or suboptimal use of antibiotics. The outbreak
of methicillin-resistant Staphylococcus aureus (MRSA) occurred over fifty years ago, is now widespread throughout the world. S.
aureus is the most common bacterial pathogen, which cause skin, soft-tissue, and endovascular infections, pneumonia, septic
arthritis, endocarditis, osteomyelitis, and sepsis. As the efficacy of currently available antibiotics is declining due to MDR, and
there is an urgent need to develop new approaches to meet this challenge. In the present study, we discovered several novel and
potent inhibitors of MDR S. aureus (EMRSA-17, EMRSA-16, MRSA-252 and Pak clinical isolates) from natural and synthetic
sources. Resistance-reversal studies at molecular level were carried out by employing flow cytometric and microscopic techniques.
Synergistic and partial synergistic effect of these compounds along with antibiotics was investigated. This work has so far resulted
in the identification of novel “helper molecules”, which can increase the efficacy of existing antibiotics to over 1000 fold. Obesity
is an emerging challenge to human well-being. Molecular cascade involve in obesity and associated disorders is not fully
understood. Proliferation of adipocytes plays in important role in the on-set and progression of obesity. Understanding the
phenomena of adipogenesis is of major importance as adipocyte dysfunction makes an important contribution to metabolic
diseases due to obesity. Differentiation of preadipocytes to adipocytes not only results in increasing number of adipocytes but also
provide a large pool of fat depots in adipose tissues. Thus one strategy to treat obesity is to reduce the adipocyte numbers and fat
content through targeting the mature adipocytes by diverse molecular activities. Among different therapeutic interventions, the
discovery of effective antiadipogenic compounds from various sources is considered to be a promising approach. Our recent
research is focused on the study of the inhibitory effects of natural and synthetic compounds such as steroids, flavonoids, terpenes
alkaloids and sulfonamides, on the proliferation of adipocytyes, in a dose dependent manner, as well as to check their effects on to
the mature adipocytes. This study has resulted in the identification of several new inhibition of adipogenesis process.