The aim of this study is to investigate the possible protective antioxidant role of the Ellagic acid (EA) on smoker and
non Smoker rats using biochemical and antioxidant approaches and to evaluate the acute and sub chronic toxicity test of Ellagic
Materials and Methods:
Twenty four Adult male Sprague-dawley (SD) rats were randomly divided in four groups (6 rats each
group). Group 1 control, Group 2 received Ellagic Acid, groups 3 and 4 were injected subcutaneously with (0.5 mg/kg)
Nicotine (Ni) for one month to convert the animals into smoker. Then, group 4 was gavaged with EA (50 mg/kg) for 30 days
using gastric tube. Animal sacrificed after 30 days. Antioxidants enzyme parameters, Superoxide Dismutase (SOD),
Malondialdehyde (MDA), Catalze (CTA), Nitric oxide (NO) levels were evaluated in serum and liver, kidney, heart
homogenate. Cotinine level was measured in serum.
Ellagic Acid induced an increase in the free radical-scavenging enzyme activity (SOD), and Catalze (CTA) levels in
non nicotinic rats. While in nicotinic rats the results demonstrated significant decrease in (SOD), and Catalze (CTA) levels. NO
level was significantly higher in Ni group compare to other groups. MDA level was higher in nicotinic group than other groups.
There was a significant increase in the cotinine levels in nicotinic group. While it is significantly decrease in Ellagic acid
This study showed that Ellagic Acid is able to significantly accelerate the activities of the antioxidant. No toxicity
of the Ellagic acid was demonstrated to a dose of 1000 mg/kg body weight.