, Mohd. Zafar
Organic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Chemistry, Kulliyyah of
Pharmacy, International Islamic University Malaysia (IIUM), 25200-Kuantan, Pahang DM, Malaysia.
Tetracera scandens Linn. (locally known as mempelas kasar) is used in folk remedies by various indigenous people to treat
rheumatism, lowering hypertension, lowering blood pressure, inflammatory diseases, hepatitis, internal pains, urinary disorders,
dysentery, child birth, sore throat, gout and diabetes. In this study, the effects of hypoletin isolated from the leaves of T.
scandens on the adipocyte differentiation and glucose regulation in 3T3-L1 adipocytes in vitro with regard to know its role in
the prevention of diabetes. The structure of hypoletin was elucidated based on spectroscopic methods including nuclear
magnetic resonance (NMR-1D and 2D), UV, IR and mass spectrometry. Treatment of hypoletin in a dose-dependent manner
was examined on induction of lipid accumulation by using Oil Red O staining and glucose regulation in 3T3-L1 adipocytes in
vitro. After 8 days, morphological changes and high lipid accumulation activity were observed in cells treated with 10 µg/mL
of hypoletin concentration (P < 0.01). In addition, the intracellular fat accumulation increased by up to 79.7% relative to MDItreated
control cells at dose 10 µg/mL. Furthermore, insulin-induced 2-deoxy-D-[3H] glucose uptake was significantly
increased (P < 0.001) in hypoletin treated cells as compared to control (DMSO cells). Since increase of lipid accumulation and
stimulation of glucose uptake into adipocytes are known to regulate the diabetes pathophysiology, results suggest that hypoletin
could be useful for the management and prevention of type 2 diabetes due to its adipocytes stimulation and glucose uptake