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Tiger milk mushroom is a unique mushroom that has number of medicinal properties. Communities in Malaysia have been
using this mushroom to treat different kinds of cancer. This study was conducted to elucidate the antiproliferation activity of
this national treasure mushroom on human colorectal cancer cells ( HCT 116). Colon cancer cells were cultured in the presence
of tiger milk mushroom for aqueous or methanol pressurized liquid extraction (PLE) extract at various concentrations for 24
hours. The percentage of cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-di phenyl tetrazolium bromide
(MTT) assay. The antioxidant capacity of both extracts was determined using Ferric reducing ability of plasma (FRAP) assay.
Our results showed that methanol PLE of Lignosus rhinocerus sclerotia was found to exhibit antiproliferative activity against
HCT 116 cells, with an IC50 of 600 μg/mL and 1200 μg/mL for aqueous PLE. The IC50 values suggested that the solvent
differences in pressurized liquid extraction greatly affected their antiproliferative activites. The mode of cell death induced by
Lignosus rhinocerus PLE was primarily apoptosis by externalization of phosphatidylserine.