Bioassay guided fractionation of an ethanol extract of leaves of Canarium sp. (Burseraceae Kunth.) led to the isolation of
scopoletin. The structure of this coumarin was elucidated based on spectroscopic methods including nuclear magnetic
resonance (NMR-1D and 2D), and mass spectrometry. Scopoletin inhibited the enzymatic activity of 5-lipooxygenase and
acetylcholinesterase with an IC50 equal to 1.76 μM and 0.27mM, respectively and confronted oxidation in the ABTS, DPPH,
FRAP and β-carotene bleaching assay with EC50 values equal to 5.62μM±0.03, 0.19mM±0.01, 0.25mM±0.03 and
0.65mM±0.07 respectively. Scopoletin being anti-inflammatory, acetylcholinesterase inhibitor and antioxidant might be of
value for the treatment of neurodegenerative diseases including Alzheimer’ diseases, Parkinson’s disease and Amyotrophic
Lateral Sclerosis. Further in vivo studies are warranted.