Overdose of paracetamol (PCM) will cause liver injury through free radical activity. Morinda citrifolia extract was found to
reduce the oxidative stress in many pathological conditions. Hence, the aim of this study was to evaluate the therapeutic
potential of polysaccharide rich fraction (PS) of M.citrifolia as hepatoprotective agent based on the assessment of liver function
test, oxidative stress and liver morphology changes. Male adult Sprague-Dawley rats were divided randomly into six groups of
control, PS. M.citrifolia 400mg/kg (PS400), PCM, PS.M.citrifolia (200mg/kg)+PCM (PS200+PCM), PS.M.citrifolia
(400mg/kg)+PCM (PS400+PCM) and silymarin+PCM. Supplementation was given for seven consecutive days of forced oral
and one hour after the last supplementation, induction of liver injury was done by given single dose of PCM (2g/kg) orally.
Following 48 hours, all rats were sacrificed, plasma and liver sample were taken immediately. Research findings showed levels
of alanin aminotransaminase (ALT), aspartate aminotransferase (AST), and total bilirubin in plasma was significantly (p<0.05)
lower in PS200+PCM and PS400+PCM compared with PCM group. Meanwhile, total protein and γ-glutamyl transferase
(GGT) of plasma in PS200+PCM and PS400+PCM group showed no significant difference (p<0.05) compared with PCM
group. Pretreatment of PS.M.citrifolia also significantly (p<0.05) increased glutathione (GSH) level, malondialdehyde (MDA)
and protein carbonyl concentration and activity of catalase (CAT) and superoxide dismutase (SOD) in hepatic tissue compared
with PCM group. Histological observation showed both PS.M.citrifolia treated groups prevent disruption of normal liver cells
architecture induced by PCM.These data suggest that polysaccharide rich fraction M.citrifolia has the therapeutic potential as
hepatoprotective agent against PCM induce liver injury.