Churanya Onlom, Yi Yang, Haji Akber Aisa, Neti Waranuch, Kornkanok Ingkaninan
Bioscreening Unit, Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences
and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
High-speed countercurrent chromatography (HSCCC) was successfully applied for the first time for the separation and
purification of two saponin glycosides i.e. shatavarin IX and asparagoside A from roots of Asparagus racemosus Willd.
Shatavarin IX was obtained from HSCCC using two-phase solvent system, chloroform-methanol-water with the lower phase as
the mobile phase. Asparagoside A was isolated using a solvent system, ethyl acetate-n-buthanol-water with the lower phase as
the mobile phase followed by the separation via Sephadex LH20. From 500 mg of crude extract, 7.3 mg of shatavarin IX and
6.4 mg of asparagoside A were obtained at purities of 93.9% and 88.3%, respectively. The chemical structures were elucidated
by ESI-MS and NMR. Shatavarin IX shows mild antifungal activity against Candida albicans.