Cynometra ramifolia Linn known as Sala plant. This plant, especially in Solo City categorized as rare plant and traditionaly
used to cures uric acid, diabetes, hypertension, and others pain. It is interesting to know the other activity by done the test to the
human cancer cell-lines. This research objective to identify the cytotoxicity effect of ethanol extract from C.ramiflora leaves
against T47D, HeLa and WiDr cancer cell lines. The cytotoxicity test of ethanol extract from C. ramiflora leaves determined by
MTT assay by calculating the level of IC50 which was based on the percentage of the cell death following the 24 hours
incubation with the extract. The results showed that ethanol extract of leave has cytotoxic effect to HeLa, T47D and WiDr celllines
with the IC50 of 163.37, 533.33 and 69.66 ppm respectively. This research indicated that the ethanol extract isolated from
C. ramiflora leaves was had a selective cytotoxicity effect to WiDr cell line.