The objective of this study was to investigate the effectiveness of superovulation under a complete absence of
the Corpus luteum (CL) and compare it with D1 protocol and traditional superovulation in cattle. Animals were divided
into three groups as following; 1- D1-protocol: Animals (n= 7) were leaved to get natural ovulation (D0), then received
FSH for the next 4 days of the cycle. GnRH was given 12h after the last dose of FSH. 2- No-CL superovulation protocol:
Animals (n=10) were synchronized and received PGF2α at D9 or D10 then classified to two subgroups (D9-sub-group
and D10-sub-group). After 36 h, all follicles (≥ 5 mm) were aspirated (D0). 3- Control: Animals (n=3) were submitted to
the conventional superovulation protocol. Blood samples were collected daily for 13 days. Progesterone (P4) and Estradiol
(E2) in plasma were measured by Enzyme immune assay (EIA).
The results showed that the number of growing follicles was significantly (P< 0.05) higher in both D9 and D10 subgroups
in comparison to the D1 protocol (25.8 ± 4.3 and 20 ± 1.9 vs. 10.9 ± 1.9 respectively). While the number of ovulated
follicle was higher in D9 sub-group than D10 sub-group, D1 protocol and control (13.8 ± 4.4 vs. 7.6 ± 3.5, 6.8 ± 1.5 and
In conclusion, the superovulation protocol with complete absent of the CL produced high number of growing follicles,
decreased variability and considered as a promising superovulation protocol.