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As CB banks (CBB) become commonplace and seek to increase the numbers and diversity of their inventory,
samples are being collected from off-site hospitals and shipped to the processing facility. CBB are concerned as to which
variables may significantly influence the collection and banking of CB for future use in transplantation and regenerative
medicine. Many CB samples are transported via airlines and questions have arisen as to whether samples may be
negatively impacted by ionizing radiation encountered during transport or during airport security screening measures.
Samples may arrive and be processed at different times during the work day, and concerns arise as to the effects of such
delays in cryopreservation. Further, although many CBB store processed samples in multiple aliquots, the numbers of
such aliquots are generally limited; raising the possibility that repeated rounds of freezing/thawing may be required for
optimal use; which could affect sample utility. Analyses were performed to ascertain any effects of low dose radiation on
CB utility, any changes in CB stem cells as a result of delays in cryopreservation, and to what end a CB sample could be
frozen, thawed and refrozen before losing utility. It was observed that CB samples are able to tolerate normal delays and
potential radiation exposures that might be routinely encountered during shipment to CBB. However, CB are only able to
undergo limited rounds of freezing and thawing while maintaining stem/progenitor cell activity.