Surface Plasmon Resonance Imaging (SPRI) is an optical technique emerged as a powerful tool for the simultaneous
monitoring of interactions of biomolecules arrayed onto gold substrates. To take fully advantage of the SPRI approach
a precise control of the fluidic of the analyte solution is imposed. The diffusion between the flowing buffer and the
analyte solution which is established within the fluidic system creates a liquid volume where a gradient in the analyte concentration
is present. Such gradient is shown to affect the kinetics parameters obtained from the SPRI response. We present
results obtained from a new delivery approach based on the use of air bubbles. The advantages offered by the new
approach are demonstrated by using two different interacting biomolecular systems: the streptavidin-biotin system and the
Ricinus Communis Agglutinin lectin-asialofetuin.