Kinetic Studies on Alcoholic Fermentation Under Substrate Inhibition Conditions Using a Bioreactor with Stirred Bed of Immobilized Yeast Cells
Anca-Irina Galaction1, Anca-Marcela Lupăşteanu2, Dan Caşcaval2, *
1 University of Medicine and Pharmacy “Gr.T. Popa” of Iasi, Faculty of Medical Bioengineering, Dept. of Medical Biotechnologies, M. Kogalniceanu 9-13, 700454, Iasi, Romania
2 Technical University “Gheorghe Asachi” of Iasi, Faculty of Chemical and Environmental Engineering, Dept. of Biochemical Engineering, D. Mangeron 71, 700050, Romania
Abstract
The studies on the alcoholic fermentation with immobilized S. cerevisiae cells on alginate using a bioreactor with stirred beds of biocatalysts indicated that the diffusion inside the biocatalyst particles attenuates or avoids the inhibitory effects, preserving the microbial activity. Using a mathematical model adequate for ethanol formation in heterogeneous systems the kinetic parameters V, KI and K’M have been estimated. Therefore, the obtained values of V and K’M were found to be higher than those corresponding to the homogeneous media under ethanol inhibition, but lower than those for homogeneous media without inhibitory fenomena.
Keywords: Stirred bed, immobilized cells, yeasts, alcoholic fermentation, kinetic.
Article Information
Article History:
Received Date: 5/3/2010
Revision Received Date: 30/3/2010
Acceptance Date: 12/4/2010
Electronic publication date: 29/4/2010
Collection year: 2010
© Galaction et al.; Licensee Bentham Open.
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (
http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
* Address correspondence to this author at the Technical University “Gheorghe Asachi” of Iasi D. Mangeron 71, 700050 Iasi, Romania; Fax: 0040232271311; E-mail: dancasca@ch.tuiasi.ro, dancasca@from.ro
Open Peer Review Details |
Manuscript submitted on 5-3-2010 |
Original Manuscript |
Kinetic Studies on Alcoholic Fermentation Under Substrate Inhibition Conditions Using a Bioreactor with Stirred Bed of Immobilized Yeast Cells |