1 Division of Animal Health, ICAR Research Complex for NEH, Barapani - 793103, India
2 Department of Microbiology, Assam University, Silchar - 788011, India
3 Department of Biotechnology, C.V.Sc, AAU, Khanapara, Assam, India
The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.
Methods and Material:
The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5’ UTR.
Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5’ UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.
Keyowords: Bovine viral diarrhea virus, Porcine, North east, Sequence analysis, Phylogenetic analysis, India.
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