Viral and Cellular Components of AAV2 Replication Compartments
Rebecca Vogel, Michael Seyffert, Bruna de Andrade Pereira , Cornel Fraefel*
Institute of Virology, University of Zurich, Winterthurerstr. 266a, CH-8057 Zurich, Switzerland
Adeno-associated virus 2 (AAV2) is a helpervirus-dependent parvovirus with a bi-phasic life cycle comprising latency in absence and lytic replication in presence of a helpervirus, such as adenovirus (Ad) or herpes simplex virus type 1 (HSV-1). Helpervirus-supported AAV2 replication takes place in replication compartments (RCs) in the cell nucleus where virus DNA replication and transcription occur. RCs consist of a defined set of helper virus-, AAV2-, and cellular proteins. Here we compare the profile of cellular proteins recruited into AAV2 RCs or identified in Rep78-associated complexes when either Ad or HSV-1 is the helpervirus, and we discuss the potential roles of some of these proteins in AAV2 and helpervirus infection.
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* Address correspondence to this author at the Institute of Virology, University of Zurich, Winterthurerstr. 266a, CH-8057 Zurich, Switzerland; Tel: +41 44 635 8713; Fax: +41 44 635 8911;