Jean Marcel Rodrigues Pinho, Fernanda Alves Dorella, Keila da Silva Coelho, Cristina Toscano Fonseca, Fernanda Caldas Cardoso, Roberto Meyer, Ricardo Wagner Dias Portela, Sergio Costa Oliveira, Anderson Miyoshi, Vasco Azevedo
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo
Horizonte - MG, Brazil.
Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis (CLA), a chronic small
ruminant’s disease. C. pseudotuberculosis Hsp60 expressed in E. coli was purified and tested as a vaccine candidate
against CLA. Immunization of BALB/c mice with recombinant Hsp60 (rHsp60) induced a significant anti-Hsp60 IgG response,
with greater production of IgG1 than of IgG2a. Cell-mediated immune responses induced by immunization were
characterized by elevated production of gamma interferon (IFN-γ) and interleukin (IL)-10, while IL-4 concentrations were
not significantly increased. Otherwise, mice challenged with 106 c.f.u. of a virulent C. pseudotuberculosis strain developed
abscesses and other signs of morbidity at the site of inoculation. The rate of survival of the animals immunized with
rHsp60 was slightly higher than that of mice immunized with PBS; however, all the animals died within two weeks after
challenge. We concluded that subcutaneous administration of rHsp60 does not induce effective protection against intraperitoneal
infection with C. pseudotuberculosis.