Open Chemistry Journal




(Discontinued)

ISSN: 1874-8422 ― Volume 8, 2021
RESEARCH ARTICLE

Evaluation of Safety, Antileishmanial, and Chemistry of Ethanolic Leaves Extracts of Seven Medicinal Plants: An In-vitro Study



Nargis Shaheen1, Naveeda Akhter Qureshi1, *, Attiya Iqbal1, Asma Ashraf1, Huma Fatima1
1 Department of Zoology, Faculty of Biological Science, Quaid-i-Azam University, Islamabad 45320, Pakistan.

Abstract

Background:

Cutaneous leishmaniasis is a neglected tropical disease that currently affects people among 98 countries and causes significant morbidity and mortality. Current chemotherapeutic intervention is unsatisfactory and has various limitations that highlight the necessity to develop safe and effective therapeutic approaches from natural products.

Objective:

The main objective of current study was the evaluation of the antileishmanial activity along with toxicity assessment of selected plant extracts.

Methods:

The ethanolic leaves extracts of selected plants were evaluated for their qualitative and quantitative phytochemical screening by standard protocols. The antioxidant potential of plant extracts was determined by total antioxidant capacity, ferric reducing power and DPPH radical scavenging assays. The cytotoxicity analysis using brine shrimp lethality assay and in-vitro antileishmanial activity against promastigotes of L. tropica (Accession# MN891719) were also evaluated.

Results:

The preliminary examination of crude extracts revealed that P. armeniaca showed the highest total phenolic and flavonoid content (279.62±5.40µgGAE/mgDW and 205.70 ±2.41µgQA/mgDW, respectively), among others. P. armeniaca showed strongest antioxidants (120.37±4.90 µgAAE/mgDW) and FRP values (278.71±1.03µgAAE/mgDW). All the plant extracts showed cytotoxicity in safety range >1000µg/ml except F. glomerata having LC50 values of 454.34 µg/ml. In the present study, P. communis and P. pashia showed some level of activity (LC50 56.68 and 60.95µg/ml respectively) while P. armeniaca demonstrated the highest antileishmanial activity (LC50 16.18µg/ml).

Conclusion:

The findings are highly encouraging so, further and extensive investigations of P. arminica should be carried out; especially bio guided fractionation to identify the active fraction and further chemical characterization of structure.

Keywords: Leishmania tropica, Antileishmanial activity, Antioxidant potential, Medicinal plants, Cutaneous leishmaniasis, Cytotoxicity.


Article Information


Identifiers and Pagination:

Year: 2020
Volume: 7
First Page: 26
Last Page: 36
Publisher Id: CHEM-7-26
DOI: 10.2174/1874842202007010026

Article History:

Received Date: 22/5/2020
Revision Received Date: 18/8/2020
Acceptance Date: 21/8/2020
Electronic publication date: 20/11/2020
Collection year: 2020

© 2020 Shaheen et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at Department of Zoology, Faculty of Biological Science, Quaid-i-Azam University, Islamabad 45320, Pakistan;
Tel: +92-051-90643201; E-mail: nqureshi@qau.edu.pk




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