Identification of Polyphenols and Anti-Oxidant Capacity of Piper
Mónica Ramos Escuderoa, D Fernando Ramos Escuderob, Connie M Remsbergc, Jody K Takemotoc, Neal M Daviesc, Jaime A Yáñez*, c
a Escuela de Postgrado en Agricultura Sostenible, Universidad Nacional Agraria de la Selva, Tingo María, Perú,
b Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina Humana, Universidad de San Martín de Porres, Lima, Perú,
c College of Pharmacy Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program, Washington State University, Pullman, WA 99164-6534, USA
Ethanol extracts of Piper aduncum L. leaves from five sub-regions in the tropical Peruvian rainforest were assessed for polyphenol content utilizing liquid chromatography coupled with mass spectrometry with electrospray ionization (LC-MS-ESI). The anti-oxidant capacity was estimated using a DPPH radical scavenging assay. Additionally, the reducing power (capacity to reduce Fe3+ to Fe2+), the capacity to scavenge hydrogen peroxide and the total polyphenol content, were assessed. Analysis led to the identification of gallic acid, catechin, chlorogenic acid, epicatechin, rutin, quercitrin, phloridzin, quercetin, and phloretin, as well as determination of potent anti-oxidant capacities by P. aduncum L.
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
* Address correspondence to this author at the Schering-Plough Research Institute, Drug Metabolism and Pharmacokinetics, 2015 Galloping Hill Road, Mail Stop K15-3 3700, Kenilworth, NJ 07033, USA; Tel: (908) 487-8777; E-mail: email@example.com