The Open Bioactive Compounds Journal




ISSN: 1874-8473 ― Volume 7, 2019
RESEARCH ARTICLE

Antidiabetic and Antioxidant Potential of GancidinW from Streptomyces Paradoxus VITALK03



Lokesh Ravi, Adhithya Ragunathan, Kannabiran Krishnan*
School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.

Abstract

Background:

The aim of the present study was to analyse the antidiabetic and antioxidant potential of GancidinW (GW) extracted from Streptomyces paradoxus VITALK03.

Materials and Methods:

Antidiabetic potential of GW was evaluated by assay of α-amylase and α-glucosidase inhibitory activity; haemoglobin glycosylation and yeast glucose uptake. The antioxidant potential of GW was assessed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization assay and superoxide assay. The inhibition of α-amylase and α-glucosidase by GW was also studied by in Silico molecular docking analysis.

Results:

GW (1mg/ml) showed 69.32% of α-amylase and 54.04% of α-glucosidase inhibitory activity. GW (1mg/ml) prevented haemoglobin glycosylation up to 30.92% and the glucose uptake by yeast cells was increased up to 64.38%. The binding interaction GW with α-amylase showed the least free binding energy of -6.09Kcal/mol and -7.53Kcal/mol with α-glucosidase by docking studies. GW also demonstrated moderate antioxidant activity in all the antioxidant assays performed.

Conclusion:

The results of this study suggests that the antidiabetic and antioxidant potential of GW can be probed further to develop GW as effective antidiabetic agent.

Keywords: GancidinW, Antioxidant, Antidiabetic activity, α-amylase, α-glucosidase, Glycosylation.


Article Information


Identifiers and Pagination:

Year: 2017
Volume: 05
First Page: 31
Last Page: 42
Publisher Id: TOBCJ-5-31
DOI: 10.2174/1874847301705010031

Article History:

Received Date: 19/06/2017
Revision Received Date: 05/09/2017
Acceptance Date: 09/09/2017
Electronic publication date: 31/10/2017
Collection year: 2017

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© 2017 Ravi et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at the School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India; Tel: 0416-2202024; E-mail: kkb@vit.ac.in



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