Induction of Apoptosis in Human Leukemic Cell Lines U937, K562 and HL-60 by Litchi chinensis Leaf Extract Via Activation of Mitochondria Mediated Caspase Cascades
Soma Royaa, Shila E. Besraa, Tripti Deb, Bisweswar Banerjeec, Joydeep Mukherjeed, Joseph R. Vedasiromoni*, a
a Drug Development Division, Indian Institute of Chemical Biology (A Unit of Council of Scientific & Industrial Research, India), 4, Raja S.C. Mullick Road, Kolkata-700032, India
b Infectious Diseases & Immunology Division, Indian Institute of Chemical Biology (A Unit of Council of Scientific & Industrial Research, India), 4, Raja S.C. Mullick Road, Kolkata-700032, India
c Department of Chemistry, K.N. College, Berhampur-742101, India
d Department of Life Science & Biotechnology, Jadavpur University, Kolkata-700032, India
Abstract
The apoptogenic activity of Litchi chinensis leaf extract (LCLE) was investigated against three human leukemic cell lines-U937, K562 and HL-60. LCLE inhibited cell growth and metabolic activity of the leukemic cells and showed characteristic features of apoptosis. Flow cytometric analysis showed appreciable number of cells in early and late apoptotic stages. While U937 and K562 cell populations were arrested in the G2-M phase, the HL-60 cell population was arrested in the G1 phase of cell cycle. LCLE induced apoptosis is mediated through mitochondrial intrinsic pathway involving the release of cytochrome c into the cytosol and activation of caspase-9 and caspase-3
Keywords: Litchi chinensis leaf extract, in vitro anti-leukemic mitochondria mediated intrinsic pathway, caspase-3, caspase-9.
Article Information
Article History:
Received Date: 9/10/2008
Revision Received Date: 17/10/2008
Acceptance Date: 22/10/2008
Electronic publication date: 14
/11/2008
Collection year: 2008
© Roy et al.; Licensee Bentham Open.
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (
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* Address correspondence to this author at the Drug Development Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata-700032, India; Fax: 91-33-2473-0284/1597; E-mail: j_rajan_49@yahoo.com, rajan@iicb.res.in
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Manuscript submitted on 9-10-2008 |
Original Manuscript |
Induction of Apoptosis in Human Leukemic Cell Lines U937, K562 and HL-60 by Litchi chinensis Leaf Extract Via Activation of Mitochondria Mediated Caspase Cascades |