1 Life Science Research Institute, Kumiai Chemical Industry Co. Ltd., Shizuoka 439-0031, Japan
Hyperglycemia has a potentially critical role in the promotion of sensitization, however, the clear mechanism of this phenomenon is unknown. The activation of NRF2 is a key event triggered by skin sensitizers. Therefore, we investigated the effects of high glucose on the activation of NRF2 by the skin sensitizers in vitro.
The involvement of glucose levels in NRF2 activation by cinnamaldehyde, a skin sensitizer, was assessed in human MCF-7 breast cancer cells under normal glucose conditions (1.0 g/L D-glucose) and high glucose conditions (4.5 g/L D-glucose).
High glucose induced the NRF2 transactivation, HMOX1 mRNA expression, and SOD-like activity. Nuclear NRF2 level was increased under high glucose conditions compared to normal glucose conditions. High glucose also enhanced the cinnamaldehyde-induced HMOX1 mRNA expression and SOD-like activity.
Oxidative stress caused by hyperglycemia induced additionally the activation of NRF2 signaling by skin sensitizers.
Keywords: Glucose, Skin sensitizer, Cinnamaldehyde, Oxidative stress, KEAP1-NRF2 signaling, ROS.
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